BACTÉRIAS ISOLADAS DE MANGUES DO RIO COCÓ E DO RIACHO DAS GUARIBAS (CE) E SEU POTENCIAL NA DEGRADAÇÃO DE DERIVADOS E CONSTITUINTES DE PETRÓLEO

K. M. Catter, R.M Cavalcante, N. S.E. Barreto, S. Saker-Sampaio, E. Hofer, R. H.S.F. Vieira

Abstract


The aim of this study was the isolation and characterization of autochthonous bacteria that are able to utilize petroleum and its derivatives both as sources of carbon and surfactant producers. A total of thirty two strains were isolated from sediment and water samples collected in two mangrove areas at Cocó River, Fortaleza-Ceará-Brazil and at Guaribas River, PecémCeará-Brazil. Seven strains (21.9%) were not identified. Twenty strains (62.5%) were identified as Gram-negative and five as Gram-positive (15.6%). These belong to seven genera: Acinetobacter (28%), Pseudomonas (20%), Staphylococcus (20%), Burkholderia (16%), Flavobacterium (8%), Aeromonas (4%) and Klebsiella (4%). All Gram-negative strains: Acinetobacter baumannii, Pseudomonas putida and Pseudomonas fluorescens were capable of producing surfactants using the drop-collapse technique for surfactant quantitation. The isolated Staphylococcus spp and Burkholderia gladioli were evaluated for their ability to degrade polycyclic aromatic hydrocarbons (PAHs) compounds. Seven PAHs namely acenaphthene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, dibenz[a,h]anthracene and benzo[g,h,i]anthracene were used to determine the PAH-degrading bacteria activity using gas chromatography (GC). Staphylococcus spp (81.2%) and Burkholderia gladioli (13.1%) showed best results for degradation of benzo[g,h,i]anthracene, whereas fluoranthene and pyrene were not degraded by any of the isolates. Little is known about the distribution of biosurfactant-producing bacteria in the environment, but culturable surfactant-producing bacteria appeared to be common both in undisturbed and contaminated sites.

Keywords


Petroleum derivatives; PAHs; Biosurfactant-producing bacteria; Mangrove.

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